- Title
- Relationship of sputum mast cells with clinical and inflammatory characteristics of asthma
- Creator
- Fricker, Michael; Qin, Ling; Niessen, Natalie; Baines, Katherine J.; McDonald, Vanessa M.; Scott, Hayley A.; Simpson, Jodie L.; Gibson, Peter G.
- Relation
- NHMRC.1078579 http://purl.org/au-research/grants/nhmrc/1078579
- Relation
- Clinical & Experimental Allergy Vol. 50, Issue 6, p. 696-707
- Publisher Link
- http://dx.doi.org/10.1111/cea.13609
- Publisher
- Wiley-Blackwell
- Resource Type
- journal article
- Date
- 2020
- Description
- Background: Mast cells (MCs) are innate immune cells that regulate atopic and non-atopic inflammation in the airways. MCs play a critical role in the pathogenesis of asthma, yet their relationship to airway and systemic inflammation and clinical characteristics of asthma is poorly understood. Objective: To quantify MCs in induced sputum samples and understand their relationship to airway and circulatory immune cells, and clinical variables in asthma. Methods: We employed flow cytometry of sputum samples to quantify MCs, basophils and other immune cells in 51 participants (45 asthma and 6 non-asthma controls). Relationship of MCs to airway (n = 45) and blood (n = 19) immune cells, participant demographics, asthma history, spirometry and airways hyperresponsiveness (AHR) to hypertonic saline was determined by correlation and comparison of cut-off–based sputum MC high vs low participants. Results: Mast cells, basophils and eosinophils were increased in asthma vs non-asthma control sputum. In asthma sputum, MCs, basophils and eosinophils were significantly intercorrelated, and MCs and basophils were elevated in participants with eosinophilic asthma. MCs and basophils, but not eosinophils, correlated with AHR. Sputum MC high asthma was characterized by an increased proportion of participants with uncontrolled asthma and reduced FEV1 and FVC. Trends towards similar clinical associations with elevated MCs were observed in a paucigranulocytic subpopulation (n = 15) lacking airway eosinophilia or neutrophilia. Receiver operator characteristic (ROC) analysis showed peripheral blood eosinophil (PBE) count predicted elevated sputum eosinophils and basophils, but not MCs. Conclusions and Clinical Relevance: Sputum MCs are elevated in asthma, and their measurement may be useful as they relate to key clinical features of asthma (spirometry, asthma control, AHR). PBE count did not predict airway MC status, suggesting direct measurement of airway MCs by sensitive methods such as flow cytometry should be further developed.
- Subject
- asthma; asthma control; SDG 3; Sustainable Development Goals; basophil; blood; eiosinophil; mast cell; neutrophil; obese; sputum; type 2 inflammation
- Identifier
- http://hdl.handle.net/1959.13/1461036
- Identifier
- uon:46094
- Identifier
- ISSN:0954-7894
- Rights
- This is the peer reviewed version of the following article: Fricker, Michael; Qin, Ling; Niessen, Natalie; Baines, Katherine J.; McDonald, Vanessa M.; Scott, Hayley A.; Simpson, Jodie L.; Gibson, Peter G. “Relationship of sputum mast cells with clinical and inflammatory characteristics of asthma.” Clinical & Experimental Allergy Vol. 50, Issue 6, p. 696-707 (2020), which has been published in final form at http://dx.doi.org/10.1111/cea.13609 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
- Language
- eng
- Full Text
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